In a double-blind cross-over pilot study comparing the effects of music tuned to 432 Hz versus 440 Hz, intriguing findings emerged. While the decrease in mean blood pressure values with 432 Hz music was not statistically significant, there was a notable and statistically significant decrease in the mean heart rate (−4.79 bpm, p = 0.05) and a slight decrease in mean respiratory rate values (1 r.a., p = 0.06) compared to 440 Hz. Additionally, subjects reported heightened focus and increased overall satisfaction after sessions with 432 Hz tuned music.
These results suggest a potential link between 432 Hz tuned music and a more pronounced decrease in heart rate, indicating a promising avenue for further exploration. The study concludes by proposing the repetition of the experiment with a larger sample size and the introduction of randomized controlled trials covering a broader range of clinical parameters to validate and expand upon these initial findings.